Uitment of leukocytes. Hence, mast cell may well be primary element for suppression of Th2 cytokines inside the AD-like skin lesions by oral administration of CP001. IgE is mediator of mast cell activation and we observed that CP001 oral administration decreased elevated blood IgE levels induced by repeated DNCB sensitization CP001 also suppressed IL-6 secretion and elevated IL-6 and IL-8 mRNA expression induced by PMA and A23187 in HMC-1. It seems that the reduction of infiltration of mast cells is related to reduce of degranulation of mast cells and maturation of eosinophils suppressing the release of different inflammatory cytokines.five. ConclusionOur present study clearly demonstrates that CP001 suppresses the progression of AD induced by DNBC. Additionally, inflammatory associated cytokine production and mRNA levels of IL-4, IL-13, IL-6, and IL-8 were suppressed by CP001. This suggests that CP001 might be a helpful candidate for the remedy of AD.Conflict of InterestsThe authors declare that there is no conflict of interests relating to the publication of this paper.Authors’ ContributionSoon Re Kim and Han-Seok Choi contributed equally to this study.Mediators of Inflammation[15] P. S. Wu, S. J. Wu, Y. H. Tsai, Y. H. Lin, and J. C. Chao, “Hot water extracted Lycium barbarum and Rehmannia glutinosa inhibit liver inflammation and fibrosis in rats,” The American Journal of Chinese Medicine, vol. 39, no. six, pp. 1173?191, 2011. [16] H. Kim, E. Lee, S. Lee, T. Shin, Y. Kim, and J. Kim, “Effect of Rehmannia glutinosa on instant sort allergic reaction,” International Journal of Immunopharmacology, vol. 20, no. 4-5, pp. 231?40, 1998. [17] H. M. Kim, C. S. An, K. Y. Jung, Y. Choo, J. Park, and S. Nam, “Rehmannia glutinosa inhibits tumour necrosis factor- and interleukin-1 secretion from mouse astrocytes,” Pharmacological Investigation, vol. 40, no. 2, pp. 171?76, 1999. [18] B. Jung, S. Cho, H.Buy261768-25-6 Koh, D.103128-76-3 Purity Han, and B.PMID:26760947 Lee, “Fermented Maesil (Prunus mume) with probiotics inhibits improvement of atopic dermatitis-like skin lesions in NC/Nga mice,” Veterinary Dermatology, vol. 21, no. 2, pp. 184?91, 2010. [19] E. Kim, H. Lee, S. K. Kim et al., “The bark of Betula platyphylla var. japonica inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice,” Journal of Ethnopharmacology, vol. 116, no. 2, pp. 270?78, 2008. [20] J.-E. Huh, J.-M. Hong, Y.-H. Baek, J.-D. Lee, D.-Y. Choi, and D.-S. Park, “Anti-inflammatory and anti-nociceptive effect of Betula platyphylla var. japonica in human interleukin-1stimulated fibroblast-like synoviocytes and in experimental animal models,” Journal of Ethnopharmacology, vol. 135, no. 1, pp. 126?34, 2011. [21] J. Y. Bae, S. S. Lim, J. S. Choi, and Y. H. Kang, “Protective actions of Rubus coreanus ethanol extract on collagenous extracellular matrix in ultraviolet-B irradiation-induced human dermal fibroblasts,” Nutritiona Study and Practice, vol. 1, no. four, pp. 279?84, 2007. [22] S. H. Ko, S. W. Choi, and S. K. Ye, “Comparison of antioxidant activities of seventy herbs which have been employed in Korean traditional medicine,” Nutritiona Analysis and Practice, vol. two, no. 3, pp. 143?51, 2008. [23] S. R. Kim, H. Choi, H. S. Seo, Y. K. Choi, Y. C. Shin, and S. Ko, “Topical application of herbal mixture extract inhibits ovalbumin- or two,4-dinitrochlorobenzene-induced atopic dermatitis,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Report ID 545497, 9 pages, 2012. [24] N. I. Denig, A. W. Hoke, and H. I. Maibach, “Irrit.
